The iron is the component of a great number of enzymes. The myoglobin,
muscular protein, contains iron, as well as the liver.
Iron is necessary for the hemoglobin production, molecule that transports
oxygen inside red globules. Their deficit in the last causes the ferropenic
anemia. High levels of iron are found in hemochromatosis, cirrhosis,
hepatitis and in increased transferrin levels.
The variation day to day is quite marked in healthy people1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.

STORAGE AND STABILITY

All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.

ADDITIONAL EQUIPMENT

-Spectrophotometer or colorimeter measuring at 562 nm.
– Matched cuvettes 1,0 cm light path.
– General laboratory equipment (Note 2).

SAMPLES

Serum or heparinized plasma.
Fee of hemolysis and separated from cells as rapidly as possible.
Stability of the sample: 2-8ºC for 7 days1.

INTERFERENCES
Hemolyzed samples are rejected, since erythrocytes contain iron and therefore
falsely elevate the serum results1,2.
A list of drugs and other interfering substances with iron determination has been
reported3,4.
NOTES
1-IRON CAL: Proceed carefully with this product because due its nature it can
get contaminated easily.
2-It is recommended to use disposable material. If glassware is used the
material should be soaking for 6 h in diluted HCl (20% v/v) and then
thoroughly rinsed with distilled water and dried before use.
3-Calibration with the aqueous standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a serum
Calibrator.
4-Use clean disposable pipette tips for its dispensation.
5-The reference values are strongly method dependent.
6-SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.