Acid phosphatase is an enzyme present in almost all weaves of the
organism, being particularly high in prostate, stomach, liver, muscle, spleen,
erythrocytes and platelets.
High levels of acid phosphatase are found in prostatic pathologies as
hypertrophy, prostatitis or carcinoma. In hematological disorders, bones or
liver diseases as well as in Paget’s or Gaucher`s diseases.
Decreased serum acid phosphatase has no clinical significance1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use.
Do not use the tablets if appears broken.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 450 nm 0,44.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 30ºC o 37ºC ( 0.1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum1
. Use only clear and unhemolyzed serum, separated from the clot as
soon as possible. Do not use plasma.
Acid phosphatase is very labile; stabilize by adding 50 L of acetic acid (R.4)
per mL of the sample. Stability: 7 days at 2-8ºC.
INTERFERENCES
Hemolysis interferes due the high concentration of acid phosphatase in red cells1
.
A list of drugs and other interfering substances with acid phosphatase
determination has been reported by Young et. al2,3
.
NOTES
SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
BIBLIOGRAPHY
1. Abbott L. et al. Acid phosphatase. Kaplan A et al. Clin Chem The C.V. Mosby
Co. St Louis. Toronto. Princeton 1984; 1079-1083.
2. Young DS. Effects of drugs on Clinical Lab. Tests, 4th ed AACC Press, 1995.
3. Young DS. Effects of disease on Clinical Lab. Tests, 4th ed AACC 2001.
4. Burtis A et al. Tietz Textbook of Clinical Chemistry, 3rd ed AACC 1999.
5. Tietz N W et al. Clinical Guide to Laboratory Tests, 3rd ed AACC 1995.
