Store at 2-8ºC
PRINCIPLE OF THE METHOD
Albumin in the presence of bromocresol green at a slightly acid pH,
produces a colour change of the indicator from yellow-green to -
green-blue. The intensity of the color formed is proportionalto the
albumin concentration in the sample1,2,3,4.
CLINICAL SIGNIFICANCE
One of the most important serum proteins produced in the liver is
albumin.
This molecule has an extraordinarily wide range of functions,
including nutrition, maintenance of oncotic pressure and transport
of Ca++, bilirubin, free fatty acid, drugs and steroids.
Variation in albumin levels indicate liver diseases, malnutrition,
skin lesions such as dermatitis and burns or dehydration1,7,8.
Clinical diagnosis should not be made on a single test result; it
should integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on
the label when stored tightly closed at 2-8ºC, protected from light
and contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 630 nm 0,40.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 630 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or plasma, free of hemolysis1: Stability 1 month at 2-8ºC or
1 week at 15-25ºC.
INTERFERENCES
Bilirubin up to 110 mg/L, hemoglobin up to 1 g/L and lipemic sera up to
10 g/L no interfere1,4.
A list of drugs and other interfering substances with albumin
determination has been reported5,6.
NOTES
1. ALBUMIN CAL: Proceed carefully with this product because due
its nature it can get contaminated easily.
2. Calibration with the aqueous Standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a
serum Calibrator.
3. Use clean disposable pipette tips for its dispensation.
4. SPINREACT has instruction sheets for several automatic
analyzers.
