Store at 2-8ºC
PRINCIPLE OF THE METHOD
Albumin in the presence of bromcresol green at a slightly acid
pH, produces a colour change of the indicator from yellowgreen to green-blue. The intensity of the color formed is
proportional to the albumin concentration in the sample1,2,3,4.
CLINICAL SIGNIFICANCE
One of the most important serum proteins produced in the
liver is albumin.
This molecule has an extraordinarily wide range of functions,
including nutrition, maintenance of oncotic pressure and
transport of Ca++, bilirubin, free fatty acid, drugs and steroids.
Variation in albumin levels indicate liver diseases,
malnutrition, skin lesions such as dermatitis and burns or
dehydration1,7,8.
Clinical diagnosis should not be made on a single test result; it
should integrate clinical and other laboratory data.
SAMPLES
Serum or plasma, free of hemolysis1: Stability 1 month at 2-8ºC or 1 week at 15-25ºC.
REFERENCE VALUES
3.5 to 5.0 g/dL1.
These values are for orientation purpose; each laboratory
should establish its own reference range.
QUALITY CONTROL
Control sera are recommended to monitor the performance of
assay procedures: SPINTROL H Normal and Pathologic (Ref.
1002120 and 1002210).
If control values are found outside the defined range, check
the instrument, reagents and calibrator for problems.
Each laboratory should establish its own Quality Control
scheme and corrective actions if controls do not meet the
acceptable tolerances.
NOTES
1. Use clean disposable pipette tips for its dispensation.
2. SPINREACT has instruction sheets for several automatic
analyzers. Instructions for many of them are available on
request.
