Store at 2-8ºC
PRINCIPLE OF THE METHOD
Albumin in the presence of bromocresol green at a slightly acid pH,
produces a colour change of the indicator from yellow-green to green-blue.
The intensity of the colour formed is proportional to the albumin
concentration in the sample1,2,3,4
CLINICAL SIGNIFICANCE
One of the most important serum proteins produced in the liver is albumin.
This molecule has an extraordinarily wide rage of functions, including
nutrition, maintenance of oncotic pressure and transport of Ca++
, bilirubin,
free fatty acid, drugs and steroids.
Variation in albumin levels indicate liver diseases, malnutrition, skin lesions
such as dermatitis and burns or dehydration1,7,8
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
REAGENTS
R Bromcresol green pH 4.2 0.12 mmol/L
PREPARATION
The reagent is ready to use.
SAMPLES
Serum or plasma, free of hemolysis1: Stability 1 month at 2-8ºC or 1 week
at 15-25ºC.
REFERENCE VALUES
3.5 to 5.0 g/dL1
These values are for orientation purpose; each laboratory should establish
its own reference range.
Conversion factor: g/dL x 144.9 = mol/L
QUALITY CONTROL
Control sera and calibrators are recommended to monitor the performance
of assay procedures: SPINTROL H Calibrator, SPINTROL H Normal and
Pathologic (Ref. 1002011, 1002120 and 1002210).
If control values are found outside the defined range, check the instrument,
reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
NOTES
1. Calibration with the aqueous Standard may cause a systematic error
in automatic procedures. In these cases, it is recommended to use a
serum Calibrator.
2. Use clean disposable pipette tips for its dispensation.
