Alkaline phosphatase is an enzyme present in almost all weaves of the
organism, being particularly high in bone, liver, placenta, intestine and
kidney.
Both increases and decreases of plasma ALP are of importance clinically.
Causes of increased plasma ALP: Paget’s disease of bone, obstructive liver
disease, hepatitis, hepatotoxicity caused by drugs or osteomalacia.
Causes of decreased plasma ALP: Cretinism and vitamin C deficiency1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use. Do not freeze the reagents.
Do not use the tablets if appears broken.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 405 nm 1,50.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0.1ºC)
- Matched cuvettes 1.0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or heparinzed plasma1
. Use unhemolyzed serum, separated from
the clot as soon as possible. Stability: 3 days at 2-8ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay procedures:
SPINTROL H Normal and Pathologic (Ref. 1002120 and 1002210).
If control values are found outside the defined range, check the instrument,
reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and corrective
actions if controls do not meet the acceptable tolerances.
INTERFERENCES
Fluoride, oxalate, citrate and EDTA inhibit alkaline phosphate activity and should
therefore not be used as anticoagulants. Haemolyses interferes due to the high
concentration of alkaline phosphatase in red cells1,2.
A list of drugs and other interfering substances with acid phosphatase
determination has been reported by Young et. al3,4.
NOTES
SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
