Calcium is the most abundant and one of the most important minerals
in the human body. Approximately 99% of body calcium is found in bones.
A decrease in albumin level causes a decrease in serum calcium. Low
levels of calcium are found in hypoparathyroidism,
pseudohypoparathyroidism, vitamin D deficiency, malnutrition and
intestinal malabsortion.
Among causes of hypercalcemia are cancers, large intake of vitamin
D, enhaced renal retention, osteoporosis, sarcosidosis, thyrotoxicosis,
hyperparathyroidism1,6,7.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations are prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 650 nm ≥ 0,50.
NOTES
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6.
CALCIUM CAL: Proceed carefully with this product because due its nature
it can get contaminated easily.
It is recommended to use disposable material. If glassware is used the
material should be scrupulously cleaned with diluted (1/2) HNO3 in water and
then thoroughly rinsed it with distilled water.
Most of the detergents and water softening products used in the
laboratories contains chelating agents. A defective rinsing will invalidate the
procedure.
Calibration with the aqueous standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a serum
Calibrator.
Use clean disposable pipette tips for its dispensation.
SPINREACT has instruction sheets for several automatic analyzers.
