It is important clinically the determination of chloride due regulation of
osmotic pressure of extra cellular fluid and to its significant role in acidbase balance.
Increases in chloride ion concentration may be found in
severe dehydratation, excessive intake of chloride, severe renal tubular
damage and in patients with cystic fibrosis.
Decrease in chloride ion concentration may be found in metabolic
acidosis, loss from prolonged vomiting and chronic pyelonephritis2,7,8.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use. Do not use reagents over
the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 505 nm
0.15.
ADDITIONAL EQUIPMENT
- SPIN 800 Autoanalyzer.
- General laboratory equipment (Note 1, 2, 3)
.
SAMPLES
- Serum or plasma free of hemolysis and separated from cells as
rapidly as possible. Anticoagulants such as oxalate or EDTA are not
acceptable they will interfere with results.
- Urine1
: Collect 24-hour urine specimen in chloride free containers.
Dilute a sample 1/2 in distilled water. Mix. Multiply results by 2 (dilution
factor).
Stability of the sample: Ion chloride is stable 1 week at room
temperature (15-25ºC), in refrigerator (2-8ºC) or frozen (-20ºC)
temperatures.
QUALITY CONTROL
Control sera and calibrators are recommended to monitor the
performance of assay procedures: SPINTROL H Calibrator,
SPINTROL H Normal and Pathologic (Ref. 1002011, 1002120 and
1002210).
If control values are found outside the defined range, check the
instrument, reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
