Cholesterol is a fat-like substance that is found in all body cells. The
liver makes all of the cholesterol the body needs to form cell
membranes and to make certain hormones.
The determination of serum cholesterol is one of the important tools in
the diagnosis and classification of lipemia. High blood cholesterol is
one of the major risk factors for heart disease5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 505 nm (500-550).
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or plasma1,2: Stability of the sample for 7 days at 2-8ºC or
freezing at –20ºC will keep samples stable for a 3 months.
INTERFERENCES
Hemoglobin up to 5 g/L and bilirubin up to 10 mg/dL, do not interfere1,2.
A list of drugs and other interfering substances with cholesterol
determination has been reported3,4.
