Cholesterol is a fat-like substance called a lipid that is found in all
body cells. The liver makes all of the cholesterol the body needs
to form cell membranes and to make certain hormones.
The determination of serum cholesterol is one of the important
tools in the diagnosis and classification of lipemia.
High blood cholesterol is one of the major risk factors for heart
disease5,6.
Clinical diagnosis should not be made on a single test result; it
should integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on
the label when stored tightly closed at 2-8ºC, protected from light
and contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 505 nm ≥ 0,26.
ADDITIONAL EQUIPMENT
Spectrophotometer or colorimeter measuring at 505 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or plasma1,2: Stability of the sample 7 days at 2-8ºC or
freezing at –20ºC will keep samples stable for 3 months.
INTERFERENCES
No interferences were observed to hemoglobin up to 5 g/L and bilirubin
up to 10 mg/dL1,2.
A list of drugs and other interfering substances with cholesterol
determination has been reported3,4.
NOTES
1-CHOLESTEROL CAL: Proceed carefully with this product because due
its nature it can get contaminated easily.
2-LCF (Lipid Clearing Factor) is integrated in the reagent.
3-Calibration with the aqueous standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a
serum Calibrator.
4-Use clean disposable pipette tips for its dispensation.
5-SPINREACT has instruction sheets for several automatic
analyzers.
