Cholinesterase is an enzyme present in plasma and synthesized by the
liver. Its true physiological function is unknown, so its function may be
to hydrolyze choline in plasma. Cholinesterase activity is usually
measured for liver function, is a sensitive test of exposure to pesticides
organophosphorus and identification of patients with the atypical form
of enzyme whose presents high sensitivity to succinyl-choline1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PREPARATION
Reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Stability: 30 days at 2-8ºC after opening, if contamination avoided.
Signs of reagent deterioration:
- Presence of particles and turbidity.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 37ºC ( 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES1,2,3,4,5
Fresh serum, plasma (EDTA heparin) not haemolyzed and promptly
separated from the red blood cells.Do not use sodium fluoride as an
anticoagulant because it inhibits cholinesterase. Stability: 7 days at 2-
8ºC or 3 months at -20ºC.
INTERFERENCES
The test is not affected by the presence of ascorbic acid up to 20 mg/dL,
haemoglobin up to 1000 mg/dL, bilirubin up to 60 mg/dL and lipids up to 1000
mg/dL.
NOTES
1. In order to avoid contamination it is recommended to use disposable
material.
2. SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
