Cholinesterase is an enzyme present in plasma and synthesized by the liver.
Its true physiological function is unknown, so its function may be to
hydrolyze choline in plasma. Cholinesterase activity is usually measured for
liver function, is a sensitive test of exposure to organophosphorus pesticides
and identification of patients with the atypical form of enzyme whose
presents high sensitivity to succinyl-choline1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PREPARATION
Working reagent (WR):
Dissolve (→) one tablet of R2 Substrate in one vial of R1.
Cap vial and mix gently to dissolve contents.
Stability: 2 hours at 2-8ºC.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use.
Do not use the tablets if appears broken.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 405 nm 1,20.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or heparinized plasma1: Stability 7 days at 2-8ºC.
INTERFERENCES
Moderate haemolysis will not interfere in the results.1,2.
A list of drugs and other interfering substances with cholinesterase determination
has been reported by Young et. al3,4.
NOTES
SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
