Cholinesterase is an enzyme present in plasma and synthesized by the liver.
Its true physiological function is unknown, so its function may be to
hydrolyze choline in plasma. Cholinesterase activity is usually measured for
liver function, is a sensitive test of exposure to pesticides organophosphorus
and identification of patients with the atypical form of enzyme whose
presents high sensitivity to succinyl-choline1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PREPARATION
Reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use.
Do not use reagents over the expiration date.
Stability: 90 days at 2-8ºC after opening, if contamination avoided and vials
recapped immediately after use.
Signs of reagent deterioration:
- Presence of particles and turbidity.
ADDITIONAL EQUIPMENT
- MINDRAY BS-120 / BS-200E Autoanalyzer.
- General laboratory equipment.
SAMPLES
Fresh serum, plasma (EDTA heparin) not haemolyzed and promptly
separated from the red blood cells.Do not use sodium fluoride as an
anticoagulant because it inhibits cholinesterase. Stability: 15 days at 2-8ºC.
REFERENCE VALUES1
Adults (37ºC) Male: 5100-11700 U/L
Female: 4000-12600 U/L
In infants up to 6 months of age, cholinesterase activity is 40% to 50% higher
than in adults. In young women, the enzyme acitvity is approximately 64%
to 74% of that in adults males. The activity decreases during pregnancy.
These values are for orientation purpose; each laboratory should establish
its own reference range.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic.
If control values are found outside the defined range, check the instrument,
reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
