CLINICAL SIGNIFICANCE
Cholinesterase is an enzyme present in plasma and synthesized by
the liver. Its true physiological function is unknown, so its function may
be to hydrolyze choline in plasma. Cholinesterase activity is usually
measured for liver function, is a sensitive test of exposure to
pesticides organophosphorus and identification of patients with the
atypical form of enzyme whose presents high sensitivity to succinylcholine1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 405 nm 1.20.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0.1ºC)
- Matched cuvettes 1.0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or heparinized or EDTA plasma1: Stability 7 days at 2-8ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic (Ref. 1002120 and
1002210).
If control values are found outside the defined range, check the instrument,
reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
Accuracy: Results obtained using SPINREACT reagents (y) did not show
systematic differences when compared with other commercial reagents (x).
The results of the performance characteristics depend on the analyzer used.
INTERFERENCES
Moderate hemolysis will not interfere in the results.1,2.
A list of drugs and
other interfering substances with cholinesterase determination has been
reported by Young et. al3,4.
NOTES
SPINREACT has instruction sheets for several autom
