Quantitative determination of copper
IVD
Store at 2-8ºC
PRINCIPLE OF THE METHOD
Direct colorimetric test without deproteinization of the sample. End point
increase. At pH 4.70, in a buffered media, copper is released from
ceruloplasmine complex and forms with the specific complexant 3-5 Di BrPAESA a stable coloured complex.
The color intensity is proportional to the amount of cooper present in the
sample4,2
CLINICAL SIGNIFICANCE
A variety of human copper deficiency conditions are recognized. Specific
diseases associated with copper include head disease, bone and joint
osteoarthritis and osteoporosis and Menkes’ syndrome, Wilson’s disease
and others. Elevated levels of copper can also be toxic.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 582 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment(Note 2).
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay procedures:
SPINTROL H Normal and Pathologic (Ref. 1002120 and 1002210).
If control values are found outside the defined range, check the instrument,
reagents and calibrator for problems.
Each laboratory should establish its own Quality Control scheme and corrective
actions if controls do not meet the acceptable tolerances.
INTERFERENCES
No interferences were observed to bilirubin up to 15 mg/dL, hemoglobin up to 0,5
g/dL and triglycerides up to 1000 mg/dL.
A list of drugs and other interfering substances with copper determination has been
reported3,4.
NOTES
1. COPPER CAL: Proceed carefully with this product because due its nature it
can get contamined easily.
2. It is recommended to use disposable material. If glassware is used the
material should be scrupulously cleaned with hydrochloric acid 1 N and then
thoroughly rinsed it with distilled water.
3. Use clean disposable pipette tips for its dispensation.
4. SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
