Glucose forms stable glycated serum proteins with several plasmatic
proteins, mainly, albumin, in covalent union. The determination of
fructosamina is based on the measurement of these glycoproteins.
The measurement of fructosamine has utility to know retrospectively (2-
3 weeks) the level of glucose concentration in blood.
This test is used for control and monitoring of diabetic patients and not
for diagnosis5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use the tablets if appears broken.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 520 0,30.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 520 nm.
- Matched cuvettes 1,0 cm light path.
- Thermostatic bath at 37ºC (±0,1ºC)
- General laboratory equipment.
SAMPLES
Serum1,2
Don’t use haemolized samples. Separated from cells as rapidly as
possible. Stability of the sample: 7 days at 2-8ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic.
If control values are found outside the defined range, check the instrument,
reagents and calibrator for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
REFERENCE VALUES
In nondiabetic samples: 187 – 287 mol/L.
These values are for orientation purpose; each laboratory should establish its
own reference range.
PERFORMANCE CHARACTERISTICS
Measuring range: From detection limit of 1,31 mol/L to linearity limit of
1000mol/L.
If the results obtained were greater than linearity limit, dilute the sample 1/2
with NaCl 9 g/L and multiply the result by 2.
INTERFERENCES
Do not interfere: Haemolysis up to 5 g/L, bilirubin up to 20 mg/dL and
triglycerides up to 6 gr/L1,2.
A list of drugs and other interfering substances with fructosamine
determination has been reported by Young et. al3,4.
NOTES
SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
