STORAGE AND STABILITY
R1 and R4 are stable until the expiration date on the label when stored tightly
closed at 2-8ºC, protected from light and contaminations prevented during their
use. R2 and R3 are stable for 4 weeks at 2-8ºC. Do not use reagents over the
expiration date.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 340 nm.
- Thermostatic bath at 37º C ( 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment (Note 1).
SAMPLES
- Erythrocytes: Preparation: Wash 0,2 ml of blood with 2 mL aliquots of 0,9%
NaCl solution. Centrifuge after each wash for 10 min at around 3000 rpm.
Repeat 3 times. Suspend the washed centrifuged erythrocytes in 0,5 mL of R4
and let stand for 15 min at +4C and then centrifuge again. Use the supernatant
in the assay within 2 hours.
INTERFERENCES
Reticulocytes have higher G-6-PDH levels than mature red cells. Therefore, it is not
recommended that this assay be performed after a severe hemolytic crisis, since G6-
PDH levels may appear falsely elevated. Testing may be more helpful after the level of
mature red cells have returned to normal.
NOTES
1. In order to avoid contamination it is recommended to use disposable material.
2. Use clean disposable pipette for its dispensation.
3. SPINREACT has instruction sheets for several automatic analyzers.
