Gamma-glutamyl transferase (γ-GT) is a cellular enzyme with wide tissue
distribution in the body, primarily in the kidney, pancreas, liver and
prostate.
Measurements of gamma-glutamyl transferase (γ-GT) activity are used in
the diagnosis and treatment of hepatobiliary diseases such biliary
obstruction, cirrhosis or liver tumours1,2,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 405 nm ≥ 1,80.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC (± 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
INTERFERENCES
Plasma should not be used, anticoagulants inhibit the enzyme. Gross haemolysis
interferes in the assay1.
A list of drugs and other interfering substances with γ-GT determination has been
reported 3,4.
NOTES
SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
