CLINICAL SIGNIFICANCE
Gamma-glutamyl transferase (γ-GT) is a cellular enzyme with wide
tissue distribution in the body, primarily in the kidney, pancreas, liver
and prostate.
Measurements of gamma-glutamyl transferase (γ-GT) activity are used
in the diagnosis and treatment of hepatobiliary diseases such biliary
obstruction, cirrhosis or liver tumours1,2,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 405 nm 1,20.
ADDITIONAL EQUIPMENT
Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum1
-γ-GT is stable for at least 3 days at 2-8ºC, 8 hours at 15-25ºC
and 1 month at – 20ºC.
INTERFERENCES
Plasma should not be used, anticoagulants inhibit the enzyme. Gross
haemolysis interferes in the assay1.
A list of drugs and other interfering substances with -GT determination
has been reported3,4.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic (Ref. 1002120 and
1002210).
If control values are found outside the defined range, check the
instrument, reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
