The AST is a cellular enzyme, is found in highest concentration in heart
muscle, the cells of the liver, the cells of the skeletal muscle and in
smaller amounts in other weaves.
Although an elevated level of AST in the serum is not specific of the
hepatic disease, is used mainly to diagnostic and to verify the course
of this disease with other enzymes like ALT and ALP.
Also, it is used to control the patients after myocardial infarction, in
skeletal muscle disease and other1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PRECAUTIONS
R1: EUH210-Safety data sheet available on request. Contains sodium
azide which can react with lead and copper plumbing, to form
potentially explosive azides. When disposing of such reagents, flush
with large volumes of water to prevent azide build up.
Follow the precautionary statements given in MSDS and label of the
product.
PREPARATION
The reagent is ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 340 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0.1ºC)
- Matched cuvettes 1.0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or plasma1: Stability 7 days at 2-8ºC.
