The AST is a cellular enzyme, is found in highest concentration in heart
muscle, the cells of the liver, the cells of the skeletal muscle and in smaller
amounts in other weaves.
Although an elevated level of AST in the serum is not specific of the hepatic
disease, is used mainly to diagnostic and to verify the course of this disease
with other enzymes like ALT and ALP.
Also it is used to control the patients after myocardial infarction, in skeletal
muscle disease and other1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PRECAUTIONS
R1: H290-May be corrosive to metals.
Follow the precautionary statements given in MSDS and label of the
product.
PREPARATION
All the reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 340 nm 1.00.
ADDITIONAL EQUIPMENT
- SPIN 800 Autoanalyzer.
- General laboratory equipment.
SAMPLES
Serum or plasma1: Stability 7 days at 2-8ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic.
If control values are found outside the defined range, check the instrument,
reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
