IFCC. Enzymatic - UV
The ALT is a cellular enzyme, found in highest concentration in liver
and kidney.
High levels are observed in hepatic disease like hepatitis, diseases of
muscles and traumatisms; its better application is in the diagnosis of
the diseases of the liver.
When they are used in conjunction with AST aid in the diagnosis of
infarcts in the myocardium, since the value of the ALT stays within the
normal limits in the presence of elevated levels of AST1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.PREPARATION
All the reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 340 nm 1.00.
ADDITIONAL EQUIPMENT
- SPIN640 / SPIN640Plus Autoanalyzer.
- General laboratory equipment.
SAMPLES
Serum or plasma1: Stability 7 days at 2-8ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic.
If control values are found outside the defined range, check the
instrument, reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
