HDL particles carry cholesterol from the cells back to the liver.
HDL is known as “good cholesterol” because high levels are
thought to lower the risk of heart disease.
A low HDL cholesterol levels, is considered a greater heart
disease risk1,6,7.
Clinical diagnosis should not be made on a single test result; it
should integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on
the label when stored tightly closed at 2-8ºC, protected from light
and contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 505 nm
(500-550).
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or plasma1: Free of hemolysis. Removed from the blood
clot as soon as possible.
Stability : HDL Cholesterol is stable for 7 days at 2-8ºC .
PROCEDURE
Precipitation (Note 1)
1. Pipette into a centrifuge tube:
2. Mix well; allow to stand for 10 min at room temperature.
3. Centrifuge at 4000 r.p.m. for 20 min or 2 min at 12000 r.p.m.
4. Collect the supernatant and proceed it as a sample in the
total cholesterol determination.
CALCULATIONS
Follow the instructions of the total cholesterol insert.
Calculated LDL-cholesterol (Friedewald)
LDLc = Total cholesterol - HDLc - (TG/5).
INTERFERENCES
No interferences were observed with triglycerides up to 4 g/L1.
A list of drugs and other interfering substances with HDL cholesterol
determination has been reported by Young et. al4,5.
NOTES
1. The Precipitation procedure can be also performed with the half of
reagent and sample volume.
2. Calibration with the aqueous Standard may cause a systematic
error in automatic procedures. In these cases, it is recommended to
use a serum Calibrator.
3. SPINREACT has instruction sheets for several automatic
analyzers. Instructions for many of them are available on
request.
