Quantitative determination of hemoglobin
IVD
Store at 2-8ºC
PRINCIPLE OF THE METHOD
Hemoglobin is oxidized by potassium ferricyanide into
metahemoglobin, which is converted into cyanometahemoglobin, by
potassium cyanide.
The intensity of the color formed is proportional to the hemoglobin
concentration in the sample1,2.
CLINICAL SIGNIFICANCE
The hemoglobin is a protein that contains iron and that the red color to
the blood. The hemoglobin is in red globules and it is the one in charge
of oxygen transport by the blood from the lungs to weaves.
When the level of hemoglobin appears underneath the normal levels is
describing an anemia that can be of different origins: primary anemia,
cancer, pregnancy, renal diseases, and hemorrhages.
If the hemoglobin levels appear high it can be due to: cardiopathies,
dehydratation and stays in places of much altitude1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 540 nm 0,012.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 540 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Venous or capillary blood1.
Use anticoagulants like EDTA, heparin or oxalate.
Stability of the sample: 1 week at 2-8ºC.
INTERFERENCES
A list of drugs and other interfering substances with hemoglobin
determination has been reported by Young et. al3,4.
