Lactate is a metabolic intermediary, originated in the lactic fermentation
from glucose, which accumulates during high intensity exercise as a
result of the associated increase in glycolytic activity. The formation of
ATP is linked to the generation of lactate and H+
If fatigue develops, the increased levels of lactate correlate with the
reduction of force1,4,5
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
SAMPLES
Plasma. Free of hemolysis1. As anticoagulants use glycolytic
inhibitors: fluoride/oxalate or fluoride/heparin.
Plasma must be placed on a refrigerator and separated of the blood
cells within 15 min; the reason is that blood cells will metabolism
glucose to lactic acid.
Once is separated, lactate is stable in plasma 8 hours at 20 – 25ºC
and 14 days at 2-8ºC.
NOTES
1. LACTATE CAL: Proceed carefully with this product because due its
nature it can get contaminated easily.
2. Calibration with the aqueous standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a serum
Calibrator.
3. Use clean disposable pipette tips for its dispensation.
4. SPINREACT has instruction sheets for several automatic
analyzers. Instructions for many of them are available on request.
