Quantitative determination of lactate dehydrogenase (LDH)
IVD
Store at 2-8ºC
CLINICAL SIGNIFICANCE
Lactate dehydrogenase (LDH) is an enzyme with wide tissue
distribution in the body.
The higher concentrations of LDH are found in liver, heart, kidney,
skeletal muscle and erythrocytes.
Increased levels of the enzyme are found in serum in liver disease,
myocardial infarction, renal disease, muscular dystrophy and
anemia1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PREPARATION
DUAL MODE: Ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 340 nm 1.00.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 340 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0.1ºC)
- Matched cuvettes 1.0 cm light path.
- General laboratory equipment.
SAMPLES
Serum1
-Separated from cells as rapidly as possible. Do not use
oxalates as anticoagulants since they inhibit the enzyme.
Do not use haemolysed samples. Stability: 2 days at 2-8ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic.
If control values are found outside the defined range, check the
instrument, reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
INTERFERENCES
Haemolysis interferes with the assay.
Some anticoagulants such as oxalates interfere with the reaction1.
A list of drugs and other interfering substances with LDH
determination has been reported2,3.
