Quantitative determination of lactate dehydrogenase
(LDH)
IVD
Store at 2-8ºC
CLINICAL SIGNIFICANCE
Lactate dehydrogenase (LDH) is an enzyme with wide tissue distribution in
the body.
The higher concentrations of LDH are found in liver, heart, kidney, skeletal
muscle and erythrocytes.
Increased levels of the enzyme are found in serum in liver disease,
myocardial infarction, renal disease, muscular dystrophy and anemia1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PRECAUTIONS
R1: H315-Causes skin irritation. H317-May cause an allergic skin reaction.
H319-Causes serious eye irritation. Contains: Proclin300.
Follow the precautionary statements given in MSDS and label of the
product.
PREPARATION
Working reagent (WR):
Mix: 4 vol. (R1) Buffer + 1 vol. (R2) Substrate
Stability: 15 days at 2-8ºC or 5 days at 15-25ºC.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 340 nm 1,00.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 340 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum1
-Separated from cells as rapidly as possible. Do not use oxalates as
anticoagulants since they inhibit the enzyme.
Do not use haemolysed samples. Stability: 2 days at 2-8ºC.
INTERFERENCES
Haemolysis interferes with the assay.
Some anticoagulants such as oxalates interfere with the reaction1.
A list of drugs and other interfering substances with LDH determination has been
reported by Young et. al2,3.
NOTES
SPINREACT has instruction sheets for several automatic analyzers.
