CLINICAL SIGNIFICANCE
Lactate dehydrogenase (LDH) is an enzyme with wide tissue
distribution in the body.
The higher concentrations of LDH are found in liver, heart, kidney,
skeletal muscle and erythrocytes.
Increased levels of the enzyme are found in serum in liver disease,
myocardial infarction, renal disease, muscular dystrophy and
anemia1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PRECAUTIONS
R1: H360- May damage fertility or the unborn child.
Follow the precautionary statements given in MSDS and label of the
product.
PREPARATION
Working reagent (WR):
Dissolve ( ) 1 tablet of R2 in one vial of R1.
Cap and mix gently to dissolve contents.
Stability: 2 days at 2-8ºC or 12 hours at room temperature (15-25ºC).
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use the tablets if appears broken.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 340 nm 1,00.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 340 nm.
- Thermostatic bath at 25ºC, 30ºC o 37ºC ( 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum1
- Separated from cells as rapidly as possible. Do not use
oxalates as anticoagulants since they inhibit the enzyme.
Do not use haemolysed samples.
Stability: 2 days at 2-8ºC.
CALCULATIONS
25º- 30ºC A/min x 4925 = U/L LDH
37ºC A/min x 9690 = U/L LDH
INTERFERENCES
Haemolysis interferes with the assay.
Some anticoagulants such as oxalates interfere with the reaction1.
A list of drugs and other interfering substances with LDH determination has
been reported by Young et. al2,3.
NOTES
SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
