Quantitative determination of lactate dehydrogenase
(LDH)
IVD
Store at 2-8ºC
The rate of decrease in concentration of NADPH, measured
photometrically, is proportional to the catalytic concentration of LDH
present in the sample1.
CLINICAL SIGNIFICANCE
Lactate dehydrogenase (LDH) is an enzyme with wide tissue
distribution in the body.
The higher concentrations of LDH are found in liver, heart, kidney,
skeletal muscle and erythrocytes.
Increased levels of the enzyme are found in serum in liver disease,
myocardial infarction, renal disease, muscular dystrophy and
anemia1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
PREPARATION
All the reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 340 nm 1,00.
ADDITIONAL EQUIPMENT
- MINDRAY BS-120 / BS-200E Autoanalyzer.
- General laboratory equipment.
SAMPLES
Serum1
Separated from cells as rapidly as possible. Do not use
oxalates as anticoagulants since they inhibit the enzyme.
Do not use haemolysed samples. Stability: 2 days at 2-8ºC.
REFERENCE VALUES1
25ºC 30ºC 37ºC
120-240 U/L 160-320 U/L 230-460 U/L
These values are for orientation purpose; each laboratory should
establish its own reference range.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic.
If control values are found outside the defined range, check the
instrument, reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
