LDLc particles are lipoproteins that transport cholesterol to the cells.
Often called “bad cholesterol” because high levels are risk factor for coronary
heart disease and are associated with obesity, diabetes and nephrosis 1,2,9.
Clinical diagnosis should not be made on a single test result; it should integrate
clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label when
stored tightly closed at 2-8ºC and contaminations are prevented during their use.
- R 1 and R 2: Once opened is stable 4 weeks at 2-8ºC.
- HDLc/LDLc CAL: Once reconstitute 30 hours at 20-25ºC, 2 weeks at 2-8ºC or
3 months –20ºC.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 600 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum, heparinized plasma or EDTA plasma. If any sample show precipitates,
centrifuge before using5.
Serum stable 6 days at 2-8ºC. Do not freeze the samples.
INTERFERENCES
The assay is unaffected by icteric samples. No interferences were observed with
ascorbic acid up to 50 mg/dL, hemoglobin up to 0,5 g/dL, bilirubin up to 30 mg/dL,
rheumatoid factors up to 1000 IU/mL or lipemic samples up to 1200 mg/dL.
Lipemic samples with a triglyceride concentration 1200 mg/dL should be diluted 1/10
with NaCl 9 g/L and multiply the result by 10.
NOTES
SPINREACT has instruction sheets for several automatic analyzers. Instructions
for many of them are available on request.
