CLINICAL SIGNIFICANCE
Lipase (LPS) is a pancreatic enzyme necessary for the absorption and digestion
of nutrients that catalyzes the hydrolysis of glycerol esters of fatty acids.
Determination of LPS is used for diagnosis of diseases of pancreas such as acute
and chronic pancreatitis and obstruction of the pancreatic duct1,7,8. Clinical
diagnosis should not be made on a single test result; it should integrate clinical
and other laboratory data.
PREPARATION
The reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label when
stored tightly closed at 2-8ºC, protected from light and contaminations prevented
during their use. Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidimetry (note 1)
- Blank absorbance (A) at 570 nm 1,00.
- R2 is a turbid orange-colored micro-emulsion, discard if turning to red.
ADDITIONAL EQUIPMENT
- SPIN 800 Autoanalyzer.
- General laboratory equipment (note 2)
SAMPLES
Fresh serum samples are recommended.
Spinreact does not recommend the use of EDTA and citrate plasma samples.
These sample types could only be used if different reference ranges are
established by each laboratory using different sample types other than fresh
serum.
Avoid repeated freezing and thawing. Stability: 14 days at 2-8ºC and at -20ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay procedures:
SPINTROL H Normal and Pathologic (Ref. 1002120 and 1002210).
If control values are found outside the defined range, check the instrument,
reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and corrective
actions if controls do not meet the acceptable tolerances.
REFERENCE VALUES
the normal range for pancreatic lipase when measured by the DGGR method is
13-60 U/L8
These values are for orientation purpose; each laboratory should establish its own
reference range.
INTERFERENCES
Bilirubin up to 75 mg/dL, hemoglobin up to 110 mg/dL and ascorbic acid up to
120 mg/dL do not interfere at the tested Lipase concentrations (40 U/L). 600
mg/dL Intralipid (1300 mg/dL triglycerides) do not interfere.
A list of drugs and other interfering substances with lipase determination has been
reported2, 3.
NOTES
1. In some storage conditions (i.e. storage at a temperature lower that the one
indicate) a precipitate may appear in the vial, it is not recommended to
resuspend the precipitate as it could alter the functionality.
2. In order to avoid contamination, it is recommended to use disposable
material.
