Enzymatic. Kinetic-Colorimetric
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2 x 10 mL / 1 x 4 mL
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4 x 10 mL / 1 x 8 mL
Lipase (LPS) is a pancreatic enzyme necessary for the absorption and digestion
of nutrients that catalyzes the hydrolysis of glycerol esters of fatty acids.
Determination of LPS is used for diagnosis of diseases of pancreas such as acute
and chronic pancreatitis and obstruction of the pancreatic duct1,7,8. Clinical
diagnosis should not be made on a single test result; it should integrate clinical
and other laboratory data.
PREPARATION
The reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label when
stored tightly closed at 2-8ºC, protected from light and contaminations prevented
during their use. Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidimetry (note 1)
- Blank absorbance (A) at 570 nm 1,00.
- R2 is a turbid orange-colored micro-emulsion, discard if turning to red.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 570 nm.
- Thermostatic bath at 37º C (± 0,1ºC)
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment (note 2)
SAMPLES
Fresh serum samples are recommended.
Spinreact does not recommend the use of EDTA and citrate plasma samples.
These sample types could only be used if different reference ranges are
established by each laboratory using different sample types other than fresh
serum.
Avoid repeated freezing and thawing. Stability: 14 days at 2-8ºC and at -20ºC
NOTES
1. In some storage conditions (i.e. storage at a temperature lower that the one
indicate) a precipitate may appear in the vial, it is not recommended to resuspend
the precipitate as it could alter the functionality.
2. In order to avoid contamination, it is recommended to use disposable material.
