PRINCIPLE OF THE METHOD
Magnesium forms a coloured complex when reacts with Magon
sulfonate in alkaline solution.
The intensity of the color formed is proportional to the magnesium
concentration in the sample1.
CLINICAL SIGNIFICANCE
Magnesium is the second more abundant intracellular cation of the
human body after potassium, being essential in great number of
enzymatic and metabolic processes.
Is a cofactor of all the enzymatic reactions that involve the ATP and
comprises of the membrane that maintains the electrical excitability of
the muscular and nervous cells.
A low magnesium level is found in malabsortion syndrome, diuretic or
aminoglucoside therapy; hyperparathyroidism or diabetic acidosis.
Elevated concentration of magnesium is found in uremia, chronic
renal failure, glomerulonephritis, Addisons’s disease or intensive anti
acid therapy1,4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
SAMPLES
Serum, heparinised plasma1:
Free of hemolysis and separated from cells as rapidly as possible.
Do not use oxalates or EDTA as anticoagulant.
Stability: 7 days at 2-8ºC.
Urine1:
Should be acidified to pH 1 with HCl.
If urine is cloudy; warm the specimen to 60ºC for 10 min. to
dissolve precipitates.
Dilute the sample 1/10 with distilled water and multiply the result by
10. Stability: 3 days at 2-8ºC.
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay procedures:
SPINTROL H Normal and Pathologic (Ref. 1002120 and 1002210).
If control values are found outside the defined range, check the instrument,
reagents and calibrator for problems.
Each laboratory should establish its own Quality Control scheme and corrective
actions if controls do not meet the acceptable tolerances.
INTERFERENCES
Hemolysis and anticoagulants other than heparin1
A list of drugs and other interfering substances with magnesium
determination has been reported 2,3.
NOTES
1. MAGNESIUM CAL: Proceed carefully with this product because due its
nature it can get contaminated easily.
2. It is recommended use disposable material to avoid magnesium
contamination. If glassware is used the material should be scrupulously
clean with H2SO4 - K2Cr2O7 and then thoroughly rinsed with distilled water
and dried before use.
3. Calibration with the aqueous standard may cause a systematic error in
automatic procedures. It is recommended to use a serum Calibrator.
4. Use clean disposable pipette tips for its dispensation.
5. SPINREACT has instruction sheets for several automatic analyzers.
