Phospholipids are a complex lipid containing phosphorus.
Their function as the principal components of cell membranes makes
phospholipids essential for all vital cell processes.
The determination of serum phospholipids is an important clinical test in
diagnosis of liver diseases, especially obstructive jaundice1,2.
Clinical diagnosis should not be made on findings of a single test result,
but should integrate both clinical and laboratory data.
PREPARATION
Working reagent (WR):
Dissolve () the contents of 1 vial R 2 Enzymes in 10 mL of R 1 Buffer.
Cap and mix gently to dissolve contents. Protect from the sunlight.
The reagent is stable after reconstitution 3 weeks at 2-8ºC or 7 days at 15-25ºC.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 505 nm 0,16.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 505 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or plasma.
Stability of the sample: 3 days at 2-8ºC.
INTERFERENCES
No influence of ascorbic acid, glucose, bilirubin, uric acid or hemoglobin was
found within the range of physiological concentration2.
A list of drugs and other interfering substances with phospholipids determination
has been reported by Young et. al3,4.
NOTES
1. PHOSPHOLIPIDS CAL: Proceed carefully with this product because due its
nature it can get contamined easily.
2. Calibration with the aqueous standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a serum
Calibrator.
3. Use clean disposable pipette tips for its dispensation.
4. SPINREACT has instruction sheets for several automatic analyzers.
Instructions for many of them are available on request.
