CLINICAL SIGNIFICANCE
In healthy individual, an extracellular fluid level of sodium is regulated
to maintain at 136 -146 mmol/L (313 -336 mg/dL1-2
). Small deviations
from normal level can have severe health consequences. Sodium has
been commonly used in the diagnosis and management of patients with
metabolic and cardiovascular disorder and is considered by American
Association of Clinical Chemistry to have the potential of severe health
consequences if left uncontrolled. Therefore monitoring serum sodium
concentration is important in both routine check and emergency rooms
CALIBRATION
This assay should be calibrated using the enclosed L and H sodium
standards. Sodium concentration in sample is determined from the
calibration curve using the included L & H sodium standards.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not freeze. Do not use reagents over the expiration date.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Thermostatic bath at 37ºC (± 0,1ºC)
- Matched cuvettes 1.0 cm light path.
- General laboratory equipment (Note 1).
SAMPLES (Note 3)
This assay is formulated for use with non hemolysed serum.
No special handling or pretreatment is needed.
Serum is the recommended sample type for this assayINTERFERENCES
The following substances normally present in serum produced less than 10%
deviation at the listed concentrations: NH4Cl at 1,5 mmol/L, KPi at 2,0 mmol/L,
CaCl2 at 7,5 mmol/L, KCl at 10 mmol/L, CuCl2 at 0,5 mmol/L, ZnCl2 at 0,5
mmol/L, FeCl3 at 0,5 mmol/L, Glucose at 5 mmol/L, ascorbate 10 mmol/L,
bilirubin at 40mg/dL, bilirubin conjugate 40 mg/dL, hemoglobin 500 mg/dL,
and triglyceride 1000 mg/dL.
NOTES
1. In order to avoid contamination it is recommended to use disposable
material.
2. Use clean disposable pipette for its dispensation.
3. When Sodium and Potassium are requested together, sodium is
assayed immediately before Potassium.
4. SPINREACT has instruction sheets for several automatic
analyzers. Instructions for many of them are available on request.
BIBLIOGRAPHY
1. Berry, M. N. et al., (1988) Clin. Chem. 34,2295
2. Tietz, N. W. (1983) Clinical guide to Laboratory Tests, p. 384 W.B.
Saunders Co., Philadelphia
