INTENDED USE
For the quantitative determination in vitro of Total Bile Acids in serum
and plasma. NAD NADH
CLINICAL SIGNIFICANCE
Fasting serum bile acids can be used in the diagnosis and prognosis of
liver disease. Levels rise in many liver diseases, for example hepatitis
and liver sclerosis. Abnormal levels in fasting patients or immediately
after a meal can be used to detect liver disease and damage, impaired
liver function, intestinal dysfunction and perhaps a gall bladder
blockage. Bile acid measurement may detect some forms of liver
disease earlier than standard liver tests because bile acids levels
correspond to liver function, rather than liver damage. In veterinary
medicine, bile acid measurement is considered to be a superior
indicator of liver disease.
PRECAUTIONS
R1 and R2 contain Sodium azide. Avoid ingestion or contact with skin
or mucous membranes. In case of skin contact, flush affected area with
copious amounts of water. In case of contact with eyes or if ingested,
seek immediate medical attention.See MSDS for disposal of the
product.
PREPARATION
Reagents are ready for use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Stability: Once opened R1 and R2 are stable for 28 days at 2-8C.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 405 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum, EDTA / Lithium heparin plasma. Serum or plasma samples
are stable for 1 week at 2-8ºC, or at 3 months at -20ºC.
INTERFERENCES
The following analytes were tested up to the levels indicated and were found
not to interfere: Haemoglobin (250 mg/dL), Triglycerides (1000 mg/dL),
Intralipid (800 mg/dL) and Bilirubin (85 mg/dL).
NOTES
1. The reagent should not be used if exposed to temperatures above 25ºC
for greater than 8 hours, as the accuracy of the assay will be affected.
2. SPINREACT has instruction sheets for several automatic
analyzers. Instructions for many of them are available on request.
BIBLIOGRAPHY
1. Komiyama, Youichi. , Adachi, Tetsuo, Ito, Yoshimasa, Hikano, Kazuyuki. ,
Sugiura, Mamoru. , Sawaki, Siiunji. Microassay Of Serum Bile Acids By An
Enzymatic Cycling Method, Chem Pharm Bull (Toyko) 30: 3796 -3797 (1982).
2. Agape, V., Russo, P., Xaiz, L., Calmi, S., and Grisler, R. Evaluation Of
Colorimetric Enzymatic Procedure for Determining The Total Bile Acids In the
Blood. Minerva Dietol Gastroenterol. Jul-Sep: 35 (3): 159 – 164 (1989).
