The proteins are macromolecular organic compounds, widely
distributed in the organism. They act like structural and transport
elements. The proteins of the serum are divide in two fractions,
albumin and globulins
The determination of total proteins is useful in the detection of:
- High protein levels caused by hemoconcentration like in the
dehydrations or increase in the concentration of specific proteins.
- Low protein level caused by hemodilution by an impared synthesis
or loss (as by hemorrhage) or excessive protein catabolism4,5.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC protected from light and
contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 540 nm ≥ 0,22.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 540 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or heparinized plasma1:
Stability of the sample: 1 month at refrigerator (2-8ºC).
QUALITY CONTROL
Control sera are recommended to monitor the performance of assay
procedures: SPINTROL H Normal and Pathologic (Ref. 1002120 and
1002210).
If control values are found outside the defined range, check the instrument,
reagents and calibrator for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
INTERFERENCES
Hemoglobin and lipemia1,4.
A list of drugs and other interfering substances with total protein
determination has been reported 2,3.
NOTES
T PROTEIN CAL: Proceed carefully with this product because due its
nature it can get contamined easily.
Calibration with the aqueous standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a
serum Calibrator.
Use clean disposable pipette tips for its dispensation.
SPINREACT has instruction sheets for several automatic
analyzers. Instructions for many of them are available on request.
