Triglycerides are fats that provide energy for the cell.
Like cholesterol, they are delivered to the body’s cells by lipoproteins in the
blood. A diet with a lot of saturated fats or carbohydrates will raise the
triglyceride levels. The increases in serum triglycerides are relatively nonspecific. For example liver dysfunction resulting from hepatitis, extra
hepatic biliary obstruction or cirrhosis, diabetes mellitus is associated with
the increase 3,6,7.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
label when stored tightly closed at 2-8ºC, protected from light and
contaminations prevented during their use. Do not use reagents over the
expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 505 nm ≥ 0,14.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 505 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment.
SAMPLES
Serum or heparinized or EDTA plasma1/ Stability of the sample: 5
days at 2-8ºC .
INTERFERENCES
No interferences were observed with bilirubin up to 170 µmol/L and
hemoglobin up to 10 g/L2
.
A list of drugs and other interfering substances with cholesterol
determination has been reported by young etal 4,5.
NOTES
1. TRIGLYCERIDES CAL: Proceed carefully with this product because due its
nature it can get contaminated easily.
2. LCF (Lipid Clearing Factor) is integrated in the reagent.
3. Calibration with the aqueous Standard may cause a systematic error in
automatic procedures. In these cases, it is recommended to use a serum
Calibrator.
4. Use clean disposable pipette tips for its dispensation.
5. SPINREACT has instruction sheets for several automatic analyzers.
