Urea is the final result of the metabolism of proteins; it is formed in the liver
from its destruction.
Elevated urea can appear in blood (uremia) in: diets with excess of proteins,
renal diseases, heart failure, gastrointestinal hemorrhage, dehydration or
renal obstruction6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the label
when stored tightly closed at 2-8ºC, protected from light and contaminations
prevented during their use.
Do not use reagents over the expiration date.
Do not use the tablets if appears broken.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 580 nm 0,32.
ADDITIONAL EQUIPMENT
- Spectrophotometer or colorimeter measuring at 580 nm.
- Matched cuvettes 1,0 cm light path.
- General laboratory equipment (Note 2).
SAMPLES
- Serum or heparinized / EDTA plasma .
- Urine3: Dilute sample 1/50 in distilled water. Mix
In all described sample types, urea is stable for at least 7 days at 2 - 8 °C
or 35 days at -20 °C.
NOTES
1. UREA CAL: Proceed carefully with this product because due its nature it can
get contaminated easily.
2. Glassware and distilled water must be free of ammonia and ammonium salts6.
3. Calibration with the aqueous standard may cause a systematic error in automatic
procedures. In these cases, it is recommended to use a serum Calibrator.
4. Use clean disposable pipette tips for its dispensation.
5. SPINREACT has instruction sheets for several automatic analyzers.